Joanne Yew, National University of Singapore
Listen Now Download as mp3 from the show eLife Episode 10: Radiation, Anti-aphrodisiacs and Glowing Squid
Pheromones are chemicals that can alter behaviour like mating. Now, researchers have discovered the pheromone equivalent of an anti-aphrodisiac. National University of Singapore scientist Joanne Yew found that male fruit flies impregnate a female fly with a cocktail of fats which puts other males off wanting to mate with her. She has been speaking to Chris Smith...
Joanne - Pheromones are chemicals that animals use to communicate with each other and we wanted to see what kinds of different chemicals are used by different species of fruit flies. So, the reason that we might be able to see something that people hadnít seen before is because of a method we worked out a couple of years ago where we use laser to scan the surface of the insects. And by doing so, weíre able to detect different kinds of molecules then those kind of classical methods that are used to identify these molecules.
Chris - So, that laser technique would enable you to study, not just what the insect itself is producing, but potentially, what another insect itís been knocking around with may have left on it.
Joanne - Yes, thatís right. And so, by using a really small laser compared to the size of the insect, we get really good spatial specificity as to where these different molecules are expressed on the surface and also, where they get transferred, following some kind of behavioural interactions like mating.
Chris - And that particular laser technique doesnít just tell you where on the insect. It will actually give you the chemical identity of the substance, will it?
Joanne - Yes. Well, it gives you a good guess. So, based on that guess, you know what is the elemental composition, what kinds of different atoms are there, and probably, number of double bonds they have.
Chris - When you started doing these with these fruit flies, what did you see that stood out as clearly different or exceptional compared to what previously people had managed to spot using traditional techniques to study pheromones?
Joanne - So, we had an expectation of the kinds of molecules you would see based on their mass. So, we have a laundry list of things to look for, but what we saw that was novel was that in males only and not females, there are series of molecules that looked a lot heavier than what had been reported before. And then when we looked at things like the elemental composition and number of double bonds, it seemed to fit characteristics of what we would expect from particular kind of fat.
Chris - So, these male flies are depositing onto the females, into the femalesí fats which appear to be linked to mating or influencing mating behaviour.
Joanne - Thatís right, yes.
Chris - So, what are these fats and how do they change the behaviour of the female, and when does the male put them on the female?
Joanne - They're transferring a particular kind of fat called triglyceride. This is a common kind of fat molecule and so, males express it in a region close to their genitals. And so, when they mate with a female, they're transferring sperm as well as these particular fats.
Chris - And the triglycerides go onto the female, but do they affect behaviour of the female or do they affect the behaviour of other males or both?
Joanne - We looked at both aspects of that. Females after they mate, they exhibit a series of rejection behaviours, where they really are not interested in mating anymore for some species. And so, if a male tries to mate with them, she will kick them in the head and run away. And so, we tried to see if we perfumed the female purely with these triglycerides and not have her mate with the male would cause her to do any of these behaviours. But it doesnít seem to be the case. So, it seems that itís more to tell other males to avoid this female.
Chris - Letís just run over that. The female that you coat in this material Ė in these triglycerides Ė does not appear to be less receptive to mating, but sheís actively avoided by males that could potentially mate with her when this chemical is present.
Joanne - Yes, thatís right.
Chris - Do you know how the males are picking it up or how they're responding to it? If they do respond like this, why donít they get confused with their own smells and therefore, just go into a non-mating state?
Joanne - Yes, thatís a great question. We speculate because itís a context-dependent. And so, they're smelling this smell on top of the femaleís natural scent. This whole bouquet of the female scent plus this added male fat is probably the signal that tells other males to avoid this female.
Chris - Now, youíve looked just in these insects in this first instance, but do you think that this whole concept Ė for want of a better phrase Ė an anti-aphrodisiac, do you think this applies in other animals too and dare I say, even in humans?
Joanne - I think so. I mean, I know so because there's actually a large body of literature showing this phenomenon in other insects. In humans, it is difficult to say especially in terms of chemical signals, but it benefits both the male and the female to have some kind of signal indicating a period where the female is not interested where males won't be successful. So, it seems reasonable that it would be a conserved mechanism.