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The Naked Scientists: Science Radio & Science Podcasts

7th Jun 2008 < Previous Show | Next Show >

The Real Ithaca and the Secrets of the Odyssey


Kat Arney

Chris Smith

 

Ancient Greece is on the naked scientists' menu this week as we travel back in time to 1200 BC to discover how modern science and a 3000 year old poem have solved an ancient riddle. A team of classicists, geologists and archaeologists claim to have found the island of Ithaca, home of the legendary Greek hero Odysseus. Digging further into the past we also hear how geophysics can help archaeologists to see what lies buried underground but without having to lift a trowel. We also learn how dormant brain stem cells can be brought back to life, why it's not just size that is important when it comes to brains, and the mind-controlling parasite that turns its host first into an egg-incubator and then into a bodyguard. Plus, in Kitchen Science, savouring the Greek flavour, Ben and Dave recreate the science of the original Naked Scientist, Archimedes, and find out whether a heap of gold coins are the real thing...

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Waking up stem cells

Many researchers are working on adult stem cells – the immortal cells that regenerate old or damaged tissues in our bodies – as they have great potential for treating many diseases, with fewer ethical issues than stem cells taken from embryos. 

Mouse embryonic stem cellsNow scientists at the Schepens Eye Research Institute in Boston have found the chemical triggers that can wake up stem cells in the brain, making them multiply and repair damaged brain areas, or send them back to sleep again.

The story started earlier this year, when the researchers, led by Dr Dong Feng Chen, found that stem cells are scattered throughout the brain, but are usually kept ‘asleep’ by chemical signals sent from neighbouring cells. 

It had previously been thought that stem cells were restricted to two specific parts of the brain, known as the subgranular zone of the hippocampus, the area responsible for learning and memory, and the subventricular zone which makes nerve cells that help us smell. So it was believed that when nerve cells died in other areas of the brain, they were lost forever.

First, the team showed that stem cells could be found all over the brain by growing tissues from different regions of mouse brains in the lab alongside supporting cells called astrocytes taken from the hippocampus (where stem cells do regenerate).  They found that nerve cells could regenerate, proving the presence of stem cells.

Next, the researchers compared the chemical signals produced by astrocytes from the hippocampus with those made by astrocytes from other areas of the brain.  They have now discovered that in areas where stem cells are ‘sleeping’, astrocytes make high levels of two molecules called ephrin-A2 and ephrin-A3. 

These molecules are produced in much lower amounts by astrocytes in the hippocampus – instead they make a protein called sonic hedgehog (named after the computer game character). The scientists have shown that removing these ephrin molecules, or adding sonic hedgehog, can reawaken stem cells.

This new research adds hope to the idea that we might be able to reactivate stem cells in damaged brains, helping to rebuild them from within.  This could potentially treat diseases such as Parkinson’s or Alzheimer’s, as well as brain or spinal cord injuries.

 The next step for the team will be to try and wake up sleeping stem cells in animals with diseases such as Parkinson’s, to see if this works in whole brains, rather than cells grown in the lab.

8th Jun 2008


Parasite turns host into bodyguard

Scientists in the Netherlands and Brazil have discovered a parasite that turns its caterpillar host first into an egg incubator and then into a bodyguard!

A caterpillar of the geometrid moth with pupae of the Braconid parasitoid waspWriting in the journal PLoS one, University of Amsterdam researcher Amir Grosman and his colleages studied how a species of parasitic wasp called Glyptapanteles takes control of the caterpillars of the geometrid moth Thyrinteina leucocerae.  The team exposed caterpillars in the laboratory to the adult wasps, which immediately laid clutches of 80 eggs inside the caterpillars bodies.  The caterpillars showed no ill effects and continued to move and eat normally for the next 11-16 days. But then things changed dramatically.

During this time the eggs had hatched inside the caterpillars and turned into larvae, which then made holes in the sides of the caterpillar and crawled out onto the nearby leaf surface where they formed a pile of pupae.  At this point the previously-parasitised caterpillar stopped moving around and also ceased eating.  Instead it took on the role of a minder and for the next 6-7 days before the adult wasps emerged it defended the pile of pupae, lashing out aggressively with head-rearing movements whenever a potential predator approached.  50% of the time at least this was sufficient to dislodge or disuade the approaching predator from lunching locally.

Unfortunately there was no reward for the hard work done by the caterpillar, which died once the adult wasps emerged from their pupae.  The team aren't sure how the wasp larvae achieve this feat of behavioural manipulation of their host, but one theory is that not all the wasp larvae vacate the caterpillar to pupate.  One or two sacrifice themselves by remaining behind, suggest the researchers, to cause the caterpillar to defend their kin, although exactly how, at this stage, remains a mystery.

8th Jun 2008


Tracing the roots of the brain

Human brains are amazing works of biological engineering, and one of our greatest challenges as scientists is to understand how they have evolved.  Now researchers writing in the journal Nature Neuroscience have shed some light on the origins of the brain, and how we developed such large, complex ones.

PET Image of the human brain showing energy consumptionCurrently, scientists think that the connections between nerve cells, known as synapses, are similar in most animals from tiny worms to humans, and that it is simply the increase in their number that allows more complicated thinking.  Put simply, the theory goes that more nerves equal more brain power.
 
But now, researchers, led by Professor Seth Grant at the Wellcome Trust Sanger Institute, have found that it is not simply size that gives us our brain power but also, during evolution, increasing complexity in the molecular processing of nerve signals at synapses.

The team looked at around 600 molecules that are found in synapses in different species, and found dramatic differences in the number of different molecules that were present.  For example, only around half are present in invertebrates (animals without a backbone, such as fruit flies) compared with higher organisms.

Remarkably, the scientists also found that around a quarter of the proteins involved in synapses (and therefore learning and memory) are even found in single-celled yeast, which obviously have no brains.  Instead, these proteins help the yeast cells to respond to signals from their environment, such as stress due to a lack of food or temperature change.

Overall, the study shows that two leaps in sophistication in the structure of nerve junctions could have been the driving force that allowed complex brains to evolve, and that this occurred before brains significantly increased in size. The first major wave of change happened around a billion years ago, when the first multicelled organisms appeared. Then the next wave happened when vertebrates came along, around 500 million years ago.

Most important for understanding of human thought, the team found the increase in synapse molecules that occurred in vertebrates provided a pool of proteins that were used for making different parts of the brain into the specialised regions such as cortex, cerebellum and spinal cord.

These new findings will be important in understanding normal functioning of the human brain and will also shed light on a number of diseases and brain problems, including mental disability.

8th Jun 2008


Finding Forgotten Fingerprints

Dr John Bond

Chris - Now, forensic scientists at the University of Leicester, working with Northamptonshire police, have announced a major breakthrough in crime detection which could lead to hundreds of cold cases being reopened.  This is the work of Dr John Bond and he’s a scientific support officer for Northamptonshire police. He’s also a fellow of Leicester University. John, thank you for joining us. You’ve found a way of getting fingerprints from surfaces that couldn’t previously be fingerprinted. How does this actually work?

A fingerprint on paperJohn - Basically, we’ve been looking at new ways of enhancing fingerprint deposit. That’s the secretion of sweat that you might leave on any surface that you might touch with your fingers. For many years the police in this country and worldwide had a range of conventional techniques but all require some sort of physical or chemical interaction with the deposit that you leave behind. They either stick to it and make it visible or they chemically react with it and maybe change its colour so you can see them.

Chris - So that must mean there are physical constraints over what sorts of surfaces can be fingerprinted?

John - Absolutely. Smooth, non-porous works very well for things like powder. A technique using superglue where the superglue actually polymerises: forms white strands on the fingerprint deposit. On things like paper where the fingerprint deposit might soak in there’s a range of chemicals that react with things like amino acids that are secreted in your sweat. All of those techniques require that deposit to still be there. If you remove the deposit all conventional techniques will fail.

Chris - This will be, for example washing the surface or wiping the surface – on the part of the criminal – to try and clean up the evidence?

John - Yes and that would be a very good example of that. It could also be extreme environment conditions that might, as you said, wash away or even vaporise the fingerprint deposit.

Chris - How does your new technique work?

Fingerprints on metalJohn - What we’ve been looking at is a phenomenon we’ve found that fingerprint deposits will tend to corrode metal surfaces. There’s some constituents in the fingerprint deposit that on metals like brass and copper will corrode the metal to an extent that even when you’ve then got rid of the residue totally you can sometimes actually see an image of where the fingerprint was in the metal or, where that’s not possible, we’ve developed a technique to actually enhance that corrosion and make the fingerprint become visible again.

Chris - So how do you visualise the fingerprint in the form of its corrosion pattern on that surface?

John - We take the metal and apply an electrical potential to it at the order of 2500V.  We then apply a very fine conducting powder, very similar to photocopying toner powder. What we’ve discovered is that that will preferentially adhere to the metal at the points where the corrosions occurred which are coincident with the original fingerprint ridge pattern. You get an image of where the fingerprint was in this black powder.

Chris - Why does it stick just where the fingerprint is? Why does it preferentially adhere there?

John - What we’ve discovered is in the areas of corrosion the potential is a few volts less than the 2500V that you apply. When the conducting powder is streaming across the surface of the metal it takes on 2500V, it takes the potential but the bulk of the metal is at. With these points of lower potential it seems to sit in that area and take the lower potentials and not have enough energy to get back up out of that potential well. It resides in the areas of lower potential.

Chris - How do you translate the photocopier toner into a physical image you can see?

John - It just appears as a black image against the contrast of the copper or the brass metal. You can actually just see it sitting there.

Chris - what sorts of things do you can apply this to which will help to solve new cases?

Fingerprint on bullet casingJohn - A very good practical example of this – and it’s very fortunate – is that most bullet casings are made of brass. Already we’ve been able to show in some of our research that a fingerprint deposited on a gun cartridge case prior to being loaded into the gun which was then enhanced after the gun has been discharged can reveal the fingerprint with this black conducting powder. For the first time we can actually get a fingerprint of who was loading the gun.

Chris - Are the police actually using this activity now or will there be a trial period before it can be admitted as evidence into say, a court of law?

John - We have demonstrated the practical use of it with these gun casings and we’ve now been approached by a number of police forces in the UK and also a prosecuting attorney. In the US that have live and sometimes historical cases with gun cartridges. People say to us,

“Look this hasn’t worked conventionally. We haven’t got anything on it. We can’t do any more. Let’s have a go with your technique.”

Chris - John, thank you very much for joining us to talk about your work.

John - Thank you.

June 2008


Does putting bananas in the fridge make them poisonous? My family have told me this but I’m sure it’s a myth. Adam

This is a bit of an old wives’ tale.  I’ve found it repeated elsewhere as well: people going, “My mother said I should never put bananas in the fridge or I’m going to die!”  Basically, this is not true.  Bananas are not poisonous and they do get refrigerated along their journey from wherever they grow, tropical places to you. Bananas produce a gas called ethylene or ethene and this is used to ripen fruit. So if you’ve got some hard fruit and you want to ripen it up stick it in a bag with a ripe banana and it will ripen nice and quickly.

One thing that will happen with bananas in the freezer is that they will go black.  Bananas have really sensitive cells in their skin, and so get damaged very easily below about 12 degrees centigrade.  They release enzymes and this is what causes that black oxidation.  The banana inside will be fresh and lovely!

June 2008


If a carbon atom is in existence at the beginning of the Earth, spends some of its time in bacteria, then in fish and animals and so on. It gets into a tree and into furniture and we try and carbon date the furniture aren’t we just carbon dating bacteria? How does it really work? Paul, New Zealand

They key to carbon dating is that the carbon isn’t the carbon that’s been on Earth ever since the Earth was formed.  The carbon that’s in carbon dating is carbon that’s been newly made.  Where that comes from is when cosmic rays - high energy particles from the sun -  hit the Earth’s atmosphere they interact with atoms and send neutrons flying around.  when one of these neutrons hit a Nitrogen-14 (14N) atom, it knocks out a proton, and the 14N becomes Carbon-14 (14C).  This then circulates in the atmosphere but because this process is happening roughly at the same rate continuously the amount of carbon that’s in the atmosphere is roughly continuous.  Most of it ends up in the atmosphere as carbon dioxide so you have 14C carbon dioxide. Plants then pick that up in their process of photosynthesis and they turn it into sugar. You then eat the plant and all the time that you are alive you’re gaining radioactive carbon in your body which you incorporate into your body. The level in your body will be roughly constant because you’re taking it in at a roughly constant rate from the environment. The ratio of radioactive to non-radioactive carbon should be the same all the time you or a plant are alive.

But when you die you stop adding new carbon-14 to your body and the 14C you’ve already got starts to break down to 14N because it’s radioactive.  The half-life is about 5500 years or so.  When you find an ancient specimen all you have to do is to compare how many 14C atoms are in it to the number of 12C atoms.  The ratio tells you how long it was since it was last alive and this gives you a ballpark figure for its age.

This does make the assumption that the production of 14C and incorporation into the food chain is the same now as it was thousands and thousands of years ago.  This assumption but it’s assumed to be a fairly reasonable and accurate way to do it.

The guy we have to credit is Willard Libby who discovered carbon dating in the 1940s, got the Nobel Prize for it actually.

June 2008


Eureka!

We recreate Archimedes' experiment to find out whether what claims to be gold is really gold, using some fairly basic equipment.

What you need

The bottle

A bottle with a tube set into the neck

Scales

Some accurate scales.

Washing up Liquid

Washing up liquid

A variety of different coins claiming to be gold.

Maple leaves

Krugerrands

Chocolate Money

If you don't have any friends who are so well endowed with gold, you could do the same experiment to compare 1 or 2p pieces which are older and younger than 1990.

What to Do

In the 3rd century BC the king Hiero II of Syracuse in Sicily had a problem, he had given a local goldsmith enough gold to make a beautiful crown for him, and the crown came back as gorgeous as promised and weighed the same as the gold he had been given.  However Hiero didn't trust the goldsmith, he thought  that the goldsmith may have swindled him by replacing some of the gold with much cheaper copper.  Hiero decided to give the job of testing the crown to the famous mathematician Archimedes who also lived in Syracuse.  We have tried to recreate his solution to this problem.

Weighing some coins

Weighing gold coins

Gold was the densest material that Archimedes knew of and it is still the 8th densest one that we know of now!

Density is the mass of a certain volume of material, so one ml of gold weighs 19.3g, but as a comparison one ml of water has a mass of about 1g.  So all Archimedes had to do was measure the density of the crown and he would know if it was Gold.

Weighing the crown was easy, however finding the volume was another matter.  If he could melt the crown down and turn it into a cube this would be trivial, but this wouldn't make the king very happy... how could he measure the volume of a complex shape like a crown?

The story goes that he was thinking about this when he was in the baths, and he noticed that when he climbed into the bath the water level rose, and some water would overflow.  If the bath started off full, the volume of water that overflowed would be the same as his volume.

So he could measure the volume of the crown by putting it in a full jug of water and measuring how much water overflowed.  He was apparently so impressed with this idea that he jumped out of the bath and ran through the streets of Syracuse entirely naked shouting "Eureka!"  Making him the first Naked Scientist.

We did the same thing, (the experiment, not the streaking) using three types of apparently gold coins.  We weighed the coins, and then measured their volumes by adding them to a bottle of water which overflowed into a cup on some weighing scales.  From the weight of water that was displaced, and knowing that 1ml of water weighs 1g, we can work out the volume of the 'gold' that has been added.

If you use pure water for this job, the water's surface tension tends to hold the water in the bottle and let it out in large rushes.  This would make the experiment less accurate, so we added washing up liquid to reduce the surface tension.

To find the coin's densities and therefore whether they were gold or not we just divided the mass of the coins by their volume.


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