Waking up stem cellsMany researchers are working on adult stem cells – the immortal cells that regenerate old or damaged tissues in our bodies – as they have great potential for treating many diseases, with fewer ethical issues than stem cells taken from embryos.
The story started earlier this year, when the researchers, led by Dr Dong Feng Chen, found that stem cells are scattered throughout the brain, but are usually kept ‘asleep’ by chemical signals sent from neighbouring cells. It had previously been thought that stem cells were restricted to two specific parts of the brain, known as the subgranular zone of the hippocampus, the area responsible for learning and memory, and the subventricular zone which makes nerve cells that help us smell. So it was believed that when nerve cells died in other areas of the brain, they were lost forever. First, the team showed that stem cells could be found all over the brain by growing tissues from different regions of mouse brains in the lab alongside supporting cells called astrocytes taken from the hippocampus (where stem cells do regenerate). They found that nerve cells could regenerate, proving the presence of stem cells. Next, the researchers compared the chemical signals produced by astrocytes from the hippocampus with those made by astrocytes from other areas of the brain. They have now discovered that in areas where stem cells are ‘sleeping’, astrocytes make high levels of two molecules called ephrin-A2 and ephrin-A3. These molecules are produced in much lower amounts by astrocytes in the hippocampus – instead they make a protein called sonic hedgehog (named after the computer game character). The scientists have shown that removing these ephrin molecules, or adding sonic hedgehog, can reawaken stem cells. This new research adds hope to the idea that we might be able to reactivate stem cells in damaged brains, helping to rebuild them from within. This could potentially treat diseases such as Parkinson’s or Alzheimer’s, as well as brain or spinal cord injuries. The next step for the team will be to try and wake up sleeping stem cells in animals with diseases such as Parkinson’s, to see if this works in whole brains, rather than cells grown in the lab. 8th Jun 2008 Parasite turns host into bodyguardScientists in the Netherlands and Brazil have discovered a parasite that turns its caterpillar host first into an egg incubator and then into a bodyguard!
During this time the eggs had hatched inside the caterpillars and turned into larvae, which then made holes in the sides of the caterpillar and crawled out onto the nearby leaf surface where they formed a pile of pupae. At this point the previously-parasitised caterpillar stopped moving around and also ceased eating. Instead it took on the role of a minder and for the next 6-7 days before the adult wasps emerged it defended the pile of pupae, lashing out aggressively with head-rearing movements whenever a potential predator approached. 50% of the time at least this was sufficient to dislodge or disuade the approaching predator from lunching locally. Unfortunately there was no reward for the hard work done by the caterpillar, which died once the adult wasps emerged from their pupae. The team aren't sure how the wasp larvae achieve this feat of behavioural manipulation of their host, but one theory is that not all the wasp larvae vacate the caterpillar to pupate. One or two sacrifice themselves by remaining behind, suggest the researchers, to cause the caterpillar to defend their kin, although exactly how, at this stage, remains a mystery.
8th Jun 2008 Tracing the roots of the brainHuman brains are amazing works of biological engineering, and one of our greatest challenges as scientists is to understand how they have evolved. Now researchers writing in the journal Nature Neuroscience have shed some light on the origins of the brain, and how we developed such large, complex ones.
The team looked at around 600 molecules that are found in synapses in different species, and found dramatic differences in the number of different molecules that were present. For example, only around half are present in invertebrates (animals without a backbone, such as fruit flies) compared with higher organisms. Remarkably, the scientists also found that around a quarter of the proteins involved in synapses (and therefore learning and memory) are even found in single-celled yeast, which obviously have no brains. Instead, these proteins help the yeast cells to respond to signals from their environment, such as stress due to a lack of food or temperature change. Overall, the study shows that two leaps in sophistication in the structure of nerve junctions could have been the driving force that allowed complex brains to evolve, and that this occurred before brains significantly increased in size. The first major wave of change happened around a billion years ago, when the first multicelled organisms appeared. Then the next wave happened when vertebrates came along, around 500 million years ago. Most important for understanding of human thought, the team found the increase in synapse molecules that occurred in vertebrates provided a pool of proteins that were used for making different parts of the brain into the specialised regions such as cortex, cerebellum and spinal cord. These new findings will be important in understanding normal functioning of the human brain and will also shed light on a number of diseases and brain problems, including mental disability. 8th Jun 2008 Finding Forgotten FingerprintsDr John BondChris - Now, forensic scientists at the University of Leicester, working with Northamptonshire police, have announced a major breakthrough in crime detection which could lead to hundreds of cold cases being reopened. This is the work of Dr John Bond and he’s a scientific support officer for Northamptonshire police. He’s also a fellow of Leicester University. John, thank you for joining us. You’ve found a way of getting fingerprints from surfaces that couldn’t previously be fingerprinted. How does this actually work?
Chris - So that must mean there are physical constraints over what sorts of surfaces can be fingerprinted? John - Absolutely. Smooth, non-porous works very well for things like powder. A technique using superglue where the superglue actually polymerises: forms white strands on the fingerprint deposit. On things like paper where the fingerprint deposit might soak in there’s a range of chemicals that react with things like amino acids that are secreted in your sweat. All of those techniques require that deposit to still be there. If you remove the deposit all conventional techniques will fail. Chris - This will be, for example washing the surface or wiping the surface – on the part of the criminal – to try and clean up the evidence? John - Yes and that would be a very good example of that. It could also be extreme environment conditions that might, as you said, wash away or even vaporise the fingerprint deposit. Chris - How does your new technique work?
Chris - So how do you visualise the fingerprint in the form of its corrosion pattern on that surface? John - We take the metal and apply an electrical potential to it at the order of 2500V. We then apply a very fine conducting powder, very similar to photocopying toner powder. What we’ve discovered is that that will preferentially adhere to the metal at the points where the corrosions occurred which are coincident with the original fingerprint ridge pattern. You get an image of where the fingerprint was in this black powder. Chris - Why does it stick just where the fingerprint is? Why does it preferentially adhere there? John - What we’ve discovered is in the areas of corrosion the potential is a few volts less than the 2500V that you apply. When the conducting powder is streaming across the surface of the metal it takes on 2500V, it takes the potential but the bulk of the metal is at. With these points of lower potential it seems to sit in that area and take the lower potentials and not have enough energy to get back up out of that potential well. It resides in the areas of lower potential. Chris - How do you translate the photocopier toner into a physical image you can see? John - It just appears as a black image against the contrast of the copper or the brass metal. You can actually just see it sitting there. Chris - what sorts of things do you can apply this to which will help to solve new cases?
Chris - Are the police actually using this activity now or will there be a trial period before it can be admitted as evidence into say, a court of law? John - We have demonstrated the practical use of it with these gun casings and we’ve now been approached by a number of police forces in the UK and also a prosecuting attorney. In the US that have live and sometimes historical cases with gun cartridges. People say to us, “Look this hasn’t worked conventionally. We haven’t got anything on it. We can’t do any more. Let’s have a go with your technique.” Chris - John, thank you very much for joining us to talk about your work. John - Thank you. June 2008
Eureka!We recreate Archimedes' experiment to find out whether what claims to be gold is really gold, using some fairly basic equipment. What you need
If you don't have any friends who are so well endowed with gold, you could do the same experiment to compare 1 or 2p pieces which are older and younger than 1990. What to DoIn the 3rd century BC the king Hiero II of Syracuse in Sicily had a problem, he had given a local goldsmith enough gold to make a beautiful crown for him, and the crown came back as gorgeous as promised and weighed the same as the gold he had been given. However Hiero didn't trust the goldsmith, he thought that the goldsmith may have swindled him by replacing some of the gold with much cheaper copper. Hiero decided to give the job of testing the crown to the famous mathematician Archimedes who also lived in Syracuse. We have tried to recreate his solution to this problem.
Gold was the densest material that Archimedes knew of and it is still the 8th densest one that we know of now! Density is the mass of a certain volume of material, so one ml of gold weighs 19.3g, but as a comparison one ml of water has a mass of about 1g. So all Archimedes had to do was measure the density of the crown and he would know if it was Gold. Weighing the crown was easy, however finding the volume was another matter. If he could melt the crown down and turn it into a cube this would be trivial, but this wouldn't make the king very happy... how could he measure the volume of a complex shape like a crown? The story goes that he was thinking about this when he was in the baths, and he noticed that when he climbed into the bath the water level rose, and some water would overflow. If the bath started off full, the volume of water that overflowed would be the same as his volume. So he could measure the volume of the crown by putting it in a full jug of water and measuring how much water overflowed. He was apparently so impressed with this idea that he jumped out of the bath and ran through the streets of Syracuse entirely naked shouting "Eureka!" Making him the first Naked Scientist. We did the same thing, (the experiment, not the streaking) using three types of apparently gold coins. We weighed the coins, and then measured their volumes by adding them to a bottle of water which overflowed into a cup on some weighing scales. From the weight of water that was displaced, and knowing that 1ml of water weighs 1g, we can work out the volume of the 'gold' that has been added. If you use pure water for this job, the water's surface tension tends to hold the water in the bottle and let it out in large rushes. This would make the experiment less accurate, so we added washing up liquid to reduce the surface tension. To find the coin's densities and therefore whether they were gold or not we just divided the mass of the coins by their volume.
What may Happen
Although our method seems to overestimate the density slightly it does show a difference between the Canadian $50 coins and the Kruegerrands (the South African coins). This is because copper is added to the Kruegerrands to make the metal harder and more resistant to damage. As copper is about half as dense as gold, the overall density is reduced.
The third set of coins had a density far too low to be gold, in fact, it is much nearer that of chocolate, which may be why they were so tasty... Why is there a difference between copper coins before and after 1992?Around this point the royal mint noticed that the price of copper was increasing rapidly and it was costing them much more than 1p to make a 1p coin. More recently the copper in a 1p coin became worth more as scrap copper than as a 1p to spend! It was a bit silly that the royal mint was making a loss when literally making money, so they decided to make the core of the coins out of steel. As banks measure an amount of pennies by weighing great big bags of them, the total weight of the new coins needed to be the same as the old ones. The coins with a steel core are less dense than than the older coins, and so need to have a larger volume in order to have the same mass. So a modern coin will be slightly thicker than an older one.
The Secrets of OdysseusProfessor James Diggle, Robert Bittlestone & Professor John UnderhillChris - Most of us have heard the tales of ancient Greece and the majority of the time we just think of these tales as another good story but there’s now a team of classicists and scientists who think the story told in one of the most famous of those ancient Greek myths, the Odyssey, might actually be based on a lot more fact than fiction. To explain how they’ve been doing this they’ve been looking into this 3000 year-old mystery as a group. We’ve got the University of Edinburgh’s Professor John Underhill and we’ve also got Robert Bittlestone who’s a businessman who also has a background in the classics and is the author of a book Odysseus Unbound, by the way. We also have Professor James Diggle. He’s the Professor of Greek and Latin at Queens’ College. James, why don’t you kick off by telling us a bit about the background to this mystery?
Chris - Is the fact that they were writing it down why they used poetry? Because there are so many rules to how you create poetry was that a way of making sure the meaning was faithful? James - Absolutely. This is a reason why you can get things that were in existence, say in Mycenaean times (1200BC) still being accurately reported, transmitted 600 years later because the oral poets used formulaic phrases which got fossilised in poetry. Once you developed a description of a place or an artefact there’s no reason to change it. Chris - If you did change it the rhyme or the meter would be wrong so they’d know that something was adrift. James. Absolutely. There was a very strict meter. Chris - How do we turn this on these ancient texts, the Odyssey and the present situation you find yourself in where you’re in this 3000 year-old mystery? James - Yes, we’re focussing on the Odyssey. Specifically the location of Odysseus’ island Ithaca. Where was it? Chris - It still exists, doesn’t it? James - Well, it would seem to many people to be a very strange question to be asking. There is indeed an island which the Greeks call Ithaki and we call Ithaca. Let’s just focus on the part of Greece that we’re talking about. We’re talking about the West coast of Greece, that is the coast that faces Italy. Halfway up that coast, right below Corfu there’s a group of three islands: Ithaki, Zakynthos and perhaps the one that’s best-known of all, Kefalonia from Captain Corelli’s Mandolin. Chris - What’s the big question? Why do we doubt that when we’re calling Ithaki Ithaca that’s what we think it really is?
First of all, the Odyssey tells us that Ithaca is in a group of four islands but modern Ithaki is one of three. Secondly, the Odyssey tells us that Ithaca is the farthest West and the farthest out to sea of these islands but Ithaki is the closest East and closest to the mainland. Finally, the Odyssey tells us that Ithaca is low-lying – that it hasn’t got any mountains but Ithaki is a mountainous island with cliffs plunging sheer into the sea. Chris - So the big question is, was the poet just using artistic license when he wrote that and just thought, “Well, it doesn’t really matter.” James - You’ve got two options. That is one. The other is to say we simply haven’t found the correct island. Given that we have found other places that are mentioned in the heroic poems like Troy and Mycenae (these have been excavated) we know that they existed. There’s therefore reason to believe that the poet described in great detail Ithaca he was talking about a real island. Chris - Let’s bring Robert in. Robert, when did you have the insight that actually we might be on the trail of the wrong island here? Robert - I was fascinated by the description that James has just given us. Here is an ancient poet describing a world in which his crucial island, the island of his hero, is farthest out to sea, farthest West, low-lying and so forth. When I looked at the map it was actually in the context of planning a family holiday some years ago. I thought, ‘how can this be?’ How can somebody get it not just marginally wrong but really, really wrong? I wondered, as you suggested, about the explanation that many, many scholars have suggested in the past. Was the reason for this that he just didn’t know or he didn’t care and he was writing a poem after all, not a travel guide? It seemed to me that that didn’t really hang together at all. The reason is it’s a motiveless crime to mis-describe your crucial island in such a way. That is to say, either you do know where it is or you don’t. If you do know where it is there’s no reason for you to mis-describe it. If you don’t know where it is why describe it so specifically but incorrectly? The very first time you recite these lines to a bunch of sailors, and Greece is a small place, they’re going to say, ‘Rubbish! It’s not there, it’s over here.’ It would be like somebody today writing a play which their lead character says without any sarcasm, ‘Oh I come from New York: a beautiful city built on a steep hill on the West coast of America.’ It’s just going to get contradicted and corrected within a very short time with its composition. That’s what drew me in. I thought this is a motiveless crime. There must be a different explanation. Chris - What did you come up with as an alternative suggestion?
Chris - A good time now to bring in John Underhill who is the Professor of Geology at Edinburgh University. He’s involved in this. Would this sit with you as something that could be a possible explanation for what was going on here? John - Yes. I first got involved with the geology of western Greece back in the 1980s when I did a PhD there. Although Thinea, this key valley that we focussed upon wasn’t a primary area as part of that it was one where I had interest and had looked at. When the opportunity materialised to get involved in the project I thought it was worthwhile given that it was a very interesting theory but inherently testable. To be honest I thought at the time that it would be easy to refute. Easy to disprove this particular theory. That’s easy to say from Scotland. When you go out into the field it actually proved much more hard to disprove the theory that Robert and James have come up with and just given to you on the ground. Chris - What they’re basically saying is that there’s been some massive ground movement which has made what was four islands become three islands. One of them is the original Ithaca. John - Yeah. The western peninsula, Peliki is very low-lying. There is a narrow valley called Thinea which separates it from the main part of Kefalonia. If that particular valley were once underwater and we’re talking 3000-2000 years ago because there are two independent references: one that James has referred to, Homeric text of the Odyssey. The second is that Strabo, the first geographer 2000 years ago (around the time of Christ he was writing) actually says that there is a narrow isthmus where Kefalonia is narrowest. From time to time, not always, it saw waters going from end-to-end. We know very specifically where he was describing because there are two Roman settlements that he mentioned within his text. They both lie on either side or at least the areas which they governed lay on either side of this valley. The theory is very challenged in this valley. We should not underestimate that. The valley itself rises to over 175m at the present day in the central saddle area. The work that I’ve undertaken around the coastlines of Kefalonia indicate that we cannot have recourse to uplift alone despite the seismicity; the earthquakes recurrence in the area. Wave-cut notches and raised beaches in the area show us that uplift is insufficient. We must appeal to other processes. Chris - What you’re saying is that we can’t just say the land has risen to join these islands together. There must be something else going on?
Chris - What have you done to test the hypothesis that these four islands have become three? John - The first thing we did was ground geology. We mapped the area and it was clear from a very early stage that the surface geology is insufficient to test the theory rigorously. You need to look beneath the ground. What you can see on the ground in the area is that there is massive landslide and rock fall debris strewn across the valley surface. Large boulders the size of houses, trucks and the like which as we know from the August ’53 earthquake, which was depicted in Captain Corelli’s Mandolin of course, there were catastrophic failures of whole hillsides at that time. They’ve been captured on film and are in the record. Chris - So you think there was a big landslide potentially that could have filled in the gap which previously was covered by ocean but filled in now by debris from a landslide? Does this mean then that you could find out historically is such a landslide existed? John - Only by looking under the ground. I think we would be foolish to think it was a single landslide. Catastrophic landslides occur regularly within this area. Indeed in November of last year without an earthquake attached to it a village, Nifi, was swept away unfortunately after a major landslide from the eastern slopes of Thinea. Tests of the principle we are looking at. Chris - But you’ve done some drilling, haven’t you? John - The first borehole that we drilled was back in Autumn of 2006. It was located on the eastern side of the valley. We drilled down from a surface elevation of 107m. We drilled down 122m so below sea level. Interestingly, that borehole found rock fall debris extending at least 40m below the current land surface i.e. 67m above present sea level. Most importantly the matrix to that borehole contained large boulders of cretaceous and other limestone material derived from the eastern slopes a very young fossil called Emiliana Huxley, a marine fossil that is 80,000 years or younger. Actually within this area we know marine waters only reached the region within the last 5000 years. A very interesting result.
Robert - I have learnt that one shouldn’t get too excited too soon as new bits of evidence come along. I think on the scale of things this is a tough geological challenge. I’m sure John would agree this isn’t something that can be solved by a field study for a couple of weeks. Yes, it was very exciting after that autumn drill hole when the borehole went right the way down, didn’t encounter any solid limestone. It encountered material that made us think with the possibility of an enormous rock fall event or series of events came thundering down the mountainside, hit a body of relatively shallow sea water and in the process of that perhaps thrust much of that water up into the air in such a way as to interpenetrate all the debris coming down and end up with all these tiny nanofossils. That’s one possible mechanism and there are other mechanisms but what we are finding as this work is done nothing John’s saying is, ‘That’s it. End of theory it’s impossible.’ It’s tough to interpret the data. It takes a lot of work, a lot of resource and fortunately a lot of industry support that we’re now getting to the project. Chris - James, you must be really pleased that this has come to this point but what do you think is going to be a next step from your classics point of view? James - I think what I find so wonderful about this is that it mixes together a whole variety of disciplines. My particular interest is on the literary and historical side but it blends with that archaeology, geology, science of various kinds, mythology – just about everything. A wonderful multidisciplinary activity that we’ve got ourselves involved in but if we can just go back to Troy and Mycenae. Troy was believed once upon a time to have never existed. Heinrich Schliemann discovered it in the 1870s. Mycenae was then excavated. Now we think we’re on the brink of demonstrating that Odysseus’ civilisation, palace on Ithaca existed too. Visit the project website: http://www.odysseus-unbound.org June 2008 Seeing Secrets UndergroundChris Leech, Geomatrix Earth Sciences LtdMeera - These days when searching for ancient remains and hidden treasures you don’t need to have the magical map where X marks the spot. You can now see into the ground using the powers of geophysics to find X for yourself. This week I’m in Leighton Buzzard in Bedfordshire to find out how to hunt for ancient remains myself. Here to help me is Chris Leech, geophysicist and director of Geomatrix Earth Sciences Ltd.
Meera - What are the techniques used? Chris L - There are several techniques which are useful for archaeological geophysics. We tend to look for the magnetic signatures of the ground. We tend to look for the electrical properties of the ground. Also we use the growing technique of ground-penetrating radar therefore looking at the electromagnetic properties of the ground. Meera - The equipment that you have strapped to yourself here is basically a horizontal pole with two vertical poles attached at either side and a rucksack that’s strapping you into it, really. Which technique does this piece of equipment use? Chris L - This is a magnetometer. Therefore we are actually measuring precisely changes in the Earth’s magnetic field at this point. The two vertical poles are actually two separate magnetometers so you can measure the Earth’s magnetic field in two places at the same time. The horizontal pole you described feeds the data back into a small data logger. By having two magnetometers we can speed up our operation. We can acquire two lines of data right at the same time. Meera - How does a magnetometer actually measure the magnetic field beneath it? Chris L - Each of the tubes which we mentioned earlier contains two fluxgate sensors. These measure very precisely the Earth’s magnetic field in the vertical component. By measuring it in two different places we can get a measure of the change in the magnetic field over that distance which is 1m between each sensor. We’re measuring the vertical gradient of the Earth’s magnetic field in two places simultaneously. Meera - How does this then translate to tell you where things are located?
Meera - So you have this magnetometer set up and strapped on, ready to go. What’s the next step to take a reading from this field that we’re in? Chris L - Well, we’ve got our grid set out. We’re at our starting point so all I need to do is press go. beep... beep... beep... Meera - So what’s happening now? Chris L - We can see the data being acquired on the screen. We can see numerical values being displayed and the beeping that you can hear is just telling us that the instrument is working. Every time it takes a reading we get the beep. Meera - How do you go from scanning an area to producing an entire map pinpointing the hotspots of where’s good to dig?
Meera - So, out in the field I just need to stick to my grid and record regular measurements to cover the whole area. I’m off to hunt for some treasure! beep... beep... beep... June 2008
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