Naked Science Forum
Life Sciences => Cells, Microbes & Viruses => Topic started by: tuhin on 30/09/2003 14:50:19
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Hello,
While performing the the total count of WBC , one needs to add a particular volume of WBC diluting fluid to a particular volume of blood . Interestingly, the WBC diluting fluid containing acetic acid (probably 2%) and Gentian violet ( to stain the nucleus of the WBCs)lyses the RBCs only ??!!
Can anyone explain this ? genegenie, there is no genetics involved, even then you should try this !!
tuhin
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Well, I may be the hick from Texas, but I'm not too proud to ask.....What's a WBC / RBC?
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WBC-white blood cell
RBC - red blood cell
Bezoar
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Ok, well I feel pretty dull. Thanks.
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Not dull at all. Medical abbreviations are a foreign abbreviation. Worse yet are the abbreviations and initials they use in aviation. They're ridiculous. Almost nothing has a full name. I felt pretty dull before I learned all of those.
Bezoar
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I was a Navy man....we had acronyms for everything. I almost forgot how to speak english. Now, medical is a whole world of latin as well as acronyms for the latin. I think sometimes it's just made up stuff. I know aviation would be tough as well.
But c'mon....WBC and RBC? I should've known that. I'm just going to beat myself up awhile over that one.
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I knew what he was talking about Ronnie[:)]
Am I dead? Am I alive? I'm both!
(https://www.thenakedscientists.com/forum/proxy.php?request=http%3A%2F%2Fwww.stupid-boy.com%2Fsmilies%2Fkao%2Fotn%2Fcat.gif&hash=e4b91a72c020cc1c5d28487fff5428f1)
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OK Ronnie, I was trying to spare your male ego, and with the truth as well, but I see Quantumcat had to rub it in. I tell you, there's no mercy!
Bezoar
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Quantumcat, way to bash that male ego[;)]! Maybe what Ian's Daddy really wants is a little "slap" from Bezoar (in her scrubs).
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Oh yes, and especially from someone old enough to be his mother. Ronnie, you pervert!
Bezoar
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Hahahahahaha. Ronnie, your name under your username should be Perverted something or other since everyone seems to know you as that now :/
Am I dead? Am I alive? I'm both!
(https://www.thenakedscientists.com/forum/proxy.php?request=http%3A%2F%2Fwww.stupid-boy.com%2Fsmilies%2Fkao%2Fotn%2Fcat.gif&hash=e4b91a72c020cc1c5d28487fff5428f1)
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Hey, do you love the way the women on this forum have attributed a reaction to Ronnie without his consent? Then, as if that's not enough, we call him names and chastize him for it. Good thing he can roll with the punches.
Bezoar
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Aw, we all know Ronnie loves attention from women, even if we are teasing him mercilessly[}:)][;)].
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I don't know tuhin....you've got me! Is it something about using a diluting fluid that's hypotonic to the RBC and causes lysis???
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What the hell!? I go out of town for a few days and I get attacked! Even though Donnah's right, I do love the female attention.[}:)]
I just want to know how it all got twisted into some sort of perversion. Was it the scrubs comment? I just like to think that I have a strong libido. Yeah, that's it...a strong libido.
Now, some are, in fact, sicker than others. I'll give you that. However, I think it's a healthy sickness on my part. Am I just dilusional?
Maybe I'm in pervert denial. Great, 12 step programs may be in my future. Maybe they have a "Men Who Dig Older Women in Scrubs Anonymous"
I'll start looking for help. Right after this good cry from all the abuse. And, if I was wrong and offended anyone, I'll gladly take my spanking.
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Ooooh, a spanking too? You are in pervert denial. Yep, it might be time for the twelve steps. What's that one, when you acknowledge that you have no control over your illness and you turn it over to a higher power? I don't think that means the nursing supervisor, with or without scrubs.
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They do say that it's a higher power as you understand it. Could be a doorknob. Could be a lampshade. Could be a spanking nurse. It's all up to the individual and what works for them.
As far as the topic, I'll take a stab and say that RBC's have a chemical make up (perhaps protiens) that are affected differently than the WBCs.
Just a thought.
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You crack me up. Man, it's your higher power, so I guess you can put her in scrubs too if you want. Just don't make any false idols of her.
You think with the diluting fluid, perhaps the chemicals react differently with the red cells because of the hemoglobin (iron) content of the cells?
Bezoar
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Exactly....on both points.
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Wow, the women on this site are really something!
I don't know much biology, but I know that the structure of WBC's is very different from the RBC's.
No words of wisdom here.
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Lysis - does that mean a loosening of the red blood cells? If so, Gentian (which is used as a circulatory aid) would theoretically be useful for RBC and cold agglutination.
Do WBCs ever stick together like RBCs sometimes do?
Tweener, welcome to the site! Why don't you get your wife to enter the forum...she just might surprise you.
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Lysis means gradual dissolution or destruction. I've never heard of anyone getting Gentian IV for cold agglutination. That's an interesting thought. I don't think white cells agglutinate like red cells, at least I don't recall hearing of it at all. With fibrinogen they will, but I think you're asking like an agglutination syndrome.
Bezoar
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Curiosity got the better of me and I looked this one up.[:I]
Apparently the 2% acetic acid is hypotonic to the RBC causing it to swell and lyse.
I'm curious now about whether WBCs can agglutinate. Does this occur in some disease states? Or maybe some infections????
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Yes, but that still doesn't explain why the WBC's don't blow up...is 2% acetate not hypotonic to them then ?
chris
"I never forget a face, but in your case I'll make an exception"
- Groucho Marx
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Ma-Donnah,
My wife constantly surprises me. I'll talk to her about this forum.
Otherwise, this thread is wayyyyy over my head.
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No words of wisdom here.
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I guess not...but I can't find anything anywhere about this.
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I'll keep looking too - I know it's a bit ott but I like the odd challenge !
We cracked the hard boiled egg conundrum in the end didn't we !
Chris
"I never forget a face, but in your case I'll make an exception"
- Groucho Marx
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Anyone know a lab tech or pathologist we could ask? I'll bet they know.
Bezoar
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Anyone know a lab tech or pathologist we could ask? I'll bet they know.
Bezoar
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hello everybody[:)],
can anyane plz let me know y at all there is rbc contamination in bonemarrow preparation?
puzzled though!
thanking u all
charu
charusanjay
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Actually both the RBC's and WBC's are lysed. However, unlike RBC's, WBC's have nuclei and those are what are counted under a microscope or even in a Coulter counter.
Peter C. Sayles
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The bone marrow is the site of red blood cell production
Peter C. Sayles
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[:)]hi ppl![:)]
ok.. so y does WBC diluting fluid lyse rbc???
the main aim is to concentrate the WBC in a given sampl of blood.. so v ve to lyse the RBCs.. and thats wat dilutin fluid does..
but y its called WBC dilutin fluid beats me.. when it actually dilutes RBCs concentration!
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I just fiinished senior level microbiology at UNCW, and we used a premixed solution labeled wright's stain, then DI water. Does that also contain acetic acid?? None of the cells were lysed for our WBC counts?