Naked Science Forum
Life Sciences => Cells, Microbes & Viruses => Topic started by: sambiotech on 07/01/2012 08:45:20
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hello all.
can any one provide me details about what house keeping gene should i use as internal control for studying TEM and HSV genes experssion study in e.coli. These genes are Beta Lactamase producing genes responsible in Efflux pump action of Beta lactum antibiotics.
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I don't really know enough about the genetic structure of E. coli to make a knowledgable contribution. But perhaps a mitrochondrialy expressive gene may prove suitable as ATPase genes are relatively ubiqitious, I beleive.
I apologise if this is erroneous, it was just a thought...
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Hey Sam,
Hopefully you've figured out a control by now... but just in case, you could try a ribosomal gene. I use those for controls in eukaryotic cell experiments and they work pretty well.
Best of luck.
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Hello,
I hope you have figured this out now :)
Common housekeeping genes used as controls are recA, rpoB, tuf... Any paper on gene expression regulation would give you the details of the genes and the related primers.