Naked Science Forum

Life Sciences => Cells, Microbes & Viruses => Topic started by: sambiotech on 07/01/2012 08:45:20

Title: Suggestion for use of internal control in gene experssion study in e.coli
Post by: sambiotech on 07/01/2012 08:45:20
hello all.

can any one provide me details about what house keeping gene should i use as internal control for studying TEM and HSV genes experssion study in e.coli. These genes are Beta Lactamase producing genes responsible in Efflux pump action of Beta lactum antibiotics.
Title: Re: Suggestion for use of internal control in gene experssion study in e.coli
Post by: Devilmunkey on 11/01/2012 21:40:00
I don't really know enough about the genetic structure of E. coli to make a knowledgable contribution. But perhaps a mitrochondrialy expressive gene may prove suitable as ATPase genes are relatively ubiqitious, I beleive.

I apologise if this is erroneous, it was just a thought...
Title: Re: Suggestion for use of internal control in gene experssion study in e.coli
Post by: virologist85 on 16/10/2012 05:33:14
Hey Sam,

Hopefully you've figured out a control by now... but just in case, you could try a ribosomal gene. I use those for controls in eukaryotic cell experiments and they work pretty well.

Best of luck.
Title: Re: Suggestion for use of internal control in gene experssion study in e.coli
Post by: Koblenz on 20/03/2013 17:27:18
Hello,

I hope you have figured this out now :)

Common housekeeping genes used as controls are recA, rpoB, tuf... Any paper on gene expression regulation would give you the details of the genes and the related primers.