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Author Topic: Culturing Pseudomonas bacteria?  (Read 4402 times)

Offline Limpet chicken

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Culturing Pseudomonas bacteria?
« on: 18/12/2004 01:03:51 »
I intend to start a culture of pseudomonas bacteria, as they contain an enzyme that I need for some experiments in production of indole-3-carboxaldehyde, but am not too experienced with bacterial cultures.

What sort of growing medium should be good to use? I imagine a warm and humid atmosphere is suitable, as Pseudomonas are commonly found in shower rooms.

Thanks.

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Offline Ylide

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Re: Culturing Pseudomonas bacteria?
« Reply #1 on: 19/12/2004 10:10:57 »
P. aeruginosa, I am assuming.  

You might want to get a microbiology textbook if you plan to culture bacteria, as it is very very easy to cross-contaminate your culture.  There are techniques that can be employed to keep your colony pure which are too involved to go into here.  You'll definately want to use a selective medium that inhibits the growth of Gram positive bacteria to reduce your contamination possibilities.  (MacConkey or EMB media should be ok)  They're aerobic, so controlling oxygen levels won't be an issue as it is with anaerobics.  

Too bad I don't still have my notes from when I took microbiology, this particular species was my pet bacteria that I cultured and experimented on all semester.

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Re: Culturing Pseudomonas bacteria?
« Reply #1 on: 19/12/2004 10:10:57 »

 

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