# The Naked Scientists Forum

### Author Topic: How do I make a 1 in 4 dilution?  (Read 18913 times)

#### abcd

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##### How do I make a 1 in 4 dilution?
« on: 27/05/2009 23:33:01 »
If I wanted to make a 1 in 4 dilution do you use 50 microlitres in 350 microlitres?
« Last Edit: 28/05/2009 04:22:35 by chris »

#### chris

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##### Re: How do I make a 1 in 4 dilution?
« Reply #1 on: 28/05/2009 04:23:07 »
How did you come up with the 350 number?

Chris

#### Chemistry4me

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##### Re: How do I make a 1 in 4 dilution?
« Reply #2 on: 28/05/2009 06:37:23 »
I don't understand your question, to make a one in four dilution, you should measure out three parts water and add to that one part stock solution, which will give a total of four parts.

#### dentstudent

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##### Re: How do I make a 1 in 4 dilution?
« Reply #3 on: 28/05/2009 08:41:12 »
If I wanted to make a 1 in 4 dilution do you use 50 microlitres in 350 microlitres?

If you added 50 into 350, that would be a 1 in 8 dilution (50/400). 50 parts in 350 is a 1 in 7 dilution (50/350). Its elementary maths. So you want a 1/4 therefore 50 should be added to 150 (50/200).

Water is always a 1 in 4 solution, it's just that the other 3 parts are water too!

#### Raghavendra

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##### Re: How do I make a 1 in 4 dilution?
« Reply #4 on: 01/06/2009 10:41:24 »
even i comeup with the doubt how did you take 350micro

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##### Re: How do I make a 1 in 4 dilution?
« Reply #4 on: 01/06/2009 10:41:24 »