The Naked Scientists

The Naked Scientists Forum

Author Topic: How can we cut DNA?  (Read 5199 times)

Offline The Scientist

  • Sr. Member
  • ****
  • Posts: 286
  • Its great to be me!
    • View Profile
How can we cut DNA?
« on: 02/07/2010 16:32:29 »
Is DNA cutting made possible with a 'scissors'? And what type of scissors would that be? thanks!


 

Offline Variola

  • Neilep Level Member
  • ******
  • Posts: 1063
  • Everyone should beware of The Pox...
    • View Profile
How can we cut DNA?
« Reply #1 on: 14/07/2010 13:47:17 »
You mean restriction enzymes? They recognise a particular sequence in the genome and will bind and digest the DNA there, very useful for cutting DNA up , or for inserting other DNA sequences in, as is common with recombineering/cloning.
 

Offline tangoblue

  • Sr. Member
  • ****
  • Posts: 301
  • RANDOMNESS RULES!!!
    • View Profile
How can we cut DNA?
« Reply #2 on: 03/08/2010 03:18:21 »
I'm sure the enzyme is lygase.
 

Offline Variola

  • Neilep Level Member
  • ******
  • Posts: 1063
  • Everyone should beware of The Pox...
    • View Profile
How can we cut DNA?
« Reply #3 on: 03/08/2010 08:39:54 »
Nearly right ligase is the enzyme that sticks bits back together  :)

Restriction enzymes  are endonuclease's  the DNA at certain points, they recognise a particular sequence, example BamH1 cuts whenever it finds the GATCC sequence in DNA.

Then if you cut a bit of DNA out, and you want to put it into a vector, say a plasmid, you use ligase to help it join up.
 

Offline chris

  • Neilep Level Member
  • ******
  • Posts: 5336
  • Thanked: 65 times
  • The Naked Scientist
    • View Profile
    • The Naked Scientists
How can we cut DNA?
« Reply #4 on: 03/08/2010 13:45:52 »
Excellent answers; thanks Variola.

Chris
 

Offline sittingoverhere

  • Jr. Member
  • **
  • Posts: 14
    • View Profile
    • Disease of the Week
How can we cut DNA?
« Reply #5 on: 04/08/2010 06:34:05 »
BamH1 is just one example. If you want to see some of the variety some biotech companies specialise in producing restriction enzymes. The list here is an example and is by no means exhaustive.
newbielink:http://www.neb.com/nebecomm/products/category1.asp [nonactive]
 

Offline Variola

  • Neilep Level Member
  • ******
  • Posts: 1063
  • Everyone should beware of The Pox...
    • View Profile
How can we cut DNA?
« Reply #6 on: 04/08/2010 08:56:12 »
Yup it is just one, but I figured it was simpler leaving it as an example, rather than going into the origins or endonuclease's and sticky vs blunt, vectors etc etc etc
 

Offline tangoblue

  • Sr. Member
  • ****
  • Posts: 301
  • RANDOMNESS RULES!!!
    • View Profile
How can we cut DNA?
« Reply #7 on: 06/08/2010 18:37:40 »
Damn, i knew it was one or the other.
 

Offline DoryT

  • Jr. Member
  • **
  • Posts: 13
    • View Profile
How can we cut DNA?
« Reply #8 on: 09/08/2010 06:11:06 »
Is restriction enzymes the only way to "cut" DNA?
 

Offline sittingoverhere

  • Jr. Member
  • **
  • Posts: 14
    • View Profile
    • Disease of the Week
How can we cut DNA?
« Reply #9 on: 11/08/2010 07:17:53 »
As far as i am aware its the only way to cut DNA with any specificity. DNA will break when exposed to tearing and shearing forces but it would happen randomly.
 

Offline DoryT

  • Jr. Member
  • **
  • Posts: 13
    • View Profile
How can we cut DNA?
« Reply #10 on: 12/08/2010 06:17:03 »
As far as i am aware its the only way to cut DNA with any specificity. DNA will break when exposed to tearing and shearing forces but it would happen randomly.

Gotcha.
 

The Naked Scientists Forum

How can we cut DNA?
« Reply #10 on: 12/08/2010 06:17:03 »

 

SMF 2.0.10 | SMF © 2015, Simple Machines
SMFAds for Free Forums