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Author Topic: WBC diluting fluid destroys RBC but not WBCs !!??  (Read 21665 times)

tuhin

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Hello,
While  performing the the total count of WBC , one needs to add a particular volume of WBC diluting fluid to a particular volume of blood . Interestingly, the WBC diluting fluid containing acetic acid (probably 2%) and Gentian violet ( to stain the nucleus of the WBCs)lyses the RBCs only ??!!

Can anyone explain this ? genegenie, there is no genetics involved, even then you should try this !!

tuhin

Ians Daddy

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #1 on: 30/09/2003 22:39:23 »
Well, I may be the hick from Texas, but I'm not too proud to ask.....What's a WBC / RBC?

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #2 on: 01/10/2003 05:02:22 »
WBC-white blood cell
RBC - red blood cell

Bezoar

Ians Daddy

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #3 on: 01/10/2003 05:56:00 »
Ok, well I feel pretty dull. Thanks.

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #4 on: 02/10/2003 04:38:45 »
Not dull at all.  Medical abbreviations are a foreign abbreviation.  Worse yet are the abbreviations and initials they use in aviation.  They're ridiculous.  Almost nothing has a full name.  I felt pretty dull before I learned all of those.

Bezoar

Ians Daddy

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #5 on: 02/10/2003 06:08:09 »
I was a Navy man....we had acronyms for everything. I almost forgot how to speak english. Now, medical is a whole world of latin as well as acronyms for the latin. I think sometimes it's just made up stuff. I know aviation would be tough as well.
But c'mon....WBC and RBC? I should've known that. I'm just going to beat myself up awhile over that one.

Quantumcat

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #6 on: 02/10/2003 08:51:26 »
I knew what he was talking about Ronnie:)

Am I dead? Am I alive? I'm both!

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #7 on: 02/10/2003 14:59:13 »
OK Ronnie, I was trying to spare your male ego, and with the truth as well, but I see Quantumcat had to rub it in.  I tell you, there's no mercy!

Bezoar

Donnah

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #8 on: 03/10/2003 00:02:38 »
Quantumcat, way to bash that male ego;)!  Maybe what Ian's Daddy really wants is a little "slap" from Bezoar (in her scrubs).

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #9 on: 03/10/2003 04:40:04 »
Oh yes, and especially from someone old enough to be his mother.  Ronnie, you pervert!

Bezoar

Quantumcat

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #10 on: 03/10/2003 08:42:49 »
Hahahahahaha. Ronnie, your name under your username should be Perverted something or other since everyone seems to know you as that now :/

Am I dead? Am I alive? I'm both!

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #11 on: 03/10/2003 18:49:38 »
Hey, do you love the way the women on this forum have attributed a reaction to Ronnie without his consent?  Then, as if that's not enough, we call him names and chastize him for it.  Good thing he can roll with the punches.

Bezoar

Donnah

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #12 on: 03/10/2003 19:23:48 »
Aw, we all know Ronnie loves attention from women, even if we are teasing him mercilessly[}:)];).
« Last Edit: 03/10/2003 19:24:16 by Donnah »

genegenie

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #13 on: 04/10/2003 13:32:50 »
I don't know tuhin....you've got me! Is it something about using a diluting fluid that's hypotonic to the RBC and causes lysis???

Ians Daddy

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #14 on: 08/10/2003 22:32:58 »
What the hell!? I go out of town for a few days and I get attacked! Even though Donnah's right, I do love the female attention.[}:)]

I just want to know how it all got twisted into some sort of perversion. Was it the scrubs comment? I just like to think that I have a strong libido. Yeah, that's it...a strong libido.

Now, some are, in fact, sicker than others. I'll give you that. However, I think it's a healthy sickness on my part. Am I just dilusional?

Maybe I'm in pervert denial. Great, 12 step programs may be in my future. Maybe they have a "Men Who Dig Older Women in Scrubs Anonymous"
I'll start looking for help. Right after this good cry from all the abuse. And, if I was wrong and offended anyone, I'll gladly take my spanking.

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #15 on: 09/10/2003 04:58:44 »
Ooooh, a spanking too?  You are in pervert denial.  Yep, it might be time for the twelve steps.  What's that one, when you acknowledge that you have no control over your illness and you turn it over to a higher power?  I don't think that means the nursing supervisor, with or without scrubs.

Ians Daddy

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #16 on: 09/10/2003 06:13:17 »
They do say that it's a higher power as you understand it. Could be a doorknob. Could be a lampshade. Could be a spanking nurse. It's all up to the individual and what works for them.

As far as the topic, I'll take a stab and say that RBC's have a chemical make up (perhaps protiens) that are affected differently than the WBCs.

Just a thought.

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #17 on: 09/10/2003 16:13:06 »
You crack me up.  Man, it's your higher power, so I guess you can put her in scrubs too if you want.  Just don't make any false idols of her.

You think with the diluting fluid, perhaps the chemicals react differently with the red cells because of the hemoglobin (iron) content of the cells?

Bezoar

Ians Daddy

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #18 on: 09/10/2003 16:58:54 »
Exactly....on both points.

tweener

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #19 on: 10/10/2003 19:03:18 »
Wow, the women on this site are really something!

I don't know much biology, but I know that the structure of WBC's is very different from the RBC's.

No words of wisdom here.

Donnah

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #20 on: 11/10/2003 01:09:14 »
Lysis - does that mean a loosening of the red blood cells?  If so, Gentian (which is used as a circulatory aid) would theoretically be useful for RBC and cold agglutination.

Do WBCs ever stick together like RBCs sometimes do?

Tweener, welcome to the site!  Why don't you get your wife to enter the forum...she just might surprise you.
« Last Edit: 11/10/2003 01:16:15 by Donnah »

bezoar

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #21 on: 11/10/2003 02:34:05 »
Lysis means gradual dissolution or destruction.  I've never heard of anyone getting Gentian IV for cold agglutination.  That's an interesting thought.  I don't think white cells agglutinate like red cells, at least I don't recall hearing of it at all.  With fibrinogen they will, but I think you're asking like an agglutination syndrome.

Bezoar

genegenie

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #22 on: 20/10/2003 13:01:37 »
Curiosity got the better of me and I looked this one up.:I
Apparently the 2% acetic acid is hypotonic to the RBC causing it to swell and lyse.

I'm curious now about whether WBCs can agglutinate. Does this occur in some disease states? Or maybe some infections????

chris

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #23 on: 20/10/2003 14:54:53 »
Yes, but that still doesn't explain why the WBC's don't blow up...is 2% acetate not hypotonic to them then ?

chris

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tweener

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Re: WBC diluting fluid destroys RBC but not WBCs !!??
« Reply #24 on: 21/10/2003 02:39:53 »
Ma-Donnah,  
My wife constantly surprises me.  I'll talk to her about this forum.

Otherwise, this thread is wayyyyy over my head.



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No words of wisdom here.

 

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