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  4. Are we able to cut a protein at any point?
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Are we able to cut a protein at any point?

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Offline scientizscht (OP)

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Are we able to cut a protein at any point?
« on: 22/06/2019 17:03:49 »
Are we able to cut a protein at any point with precision?

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Offline Bored chemist

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Re: Are we able to cut a protein at any point?
« Reply #1 on: 22/06/2019 22:03:56 »
Define "with precision".
We have some enzymes that will break a peptide chain at a specific type of bond
". Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline." (from wiki)

And there are some very specific ones
https://en.wikipedia.org/wiki/Thrombin
which cleaves chains where the pattern Leu-Val-Pro-Arg-Gly-Ser occurs
But even those would be "fooled" by a protein that has the same sequence twice.
You couldn't specify which one it cleaved.
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Offline evan_au

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Re: Are we able to cut a protein at any point?
« Reply #2 on: 22/06/2019 23:52:35 »
Quote
Are we able to cut a protein at any point?
Proteins fold in a complex 3D shape, composed of sheets, levers, hinges and pockets.

The "inside" of this structure is protected from access, so no, that part of the protein can't be cut without breaking apart other parts of the protein first.
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