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Cells, Microbes & Viruses / Re: Why didn't I find gene editing in my wheat plants?
« on: 15/12/2017 23:27:43 »
A few ideas spring to mind:
1) Maybe the edited cells were no-viable and were lost to follow up?
2) Maybe the strand-breaks introduced by CRISPR were repaired using a complementary sequence from the other parallel genome, as you surmise, rather than the guide sequence?
- there is a precedent for a strange thing like 2) happening:
When scientists "fixed" human embryos carrying a defect in a gene linked to a form of developmental heart condition, they found that the error was fixed from the complementary region of the corresponding healthy chromosome, rather than the supplied patch. Might this happening in your plants?
https://www.thenakedscientists.com/articles/science-news/gene-editing-corrects-embryonic-heart-defect
1) Maybe the edited cells were no-viable and were lost to follow up?
2) Maybe the strand-breaks introduced by CRISPR were repaired using a complementary sequence from the other parallel genome, as you surmise, rather than the guide sequence?
- there is a precedent for a strange thing like 2) happening:
When scientists "fixed" human embryos carrying a defect in a gene linked to a form of developmental heart condition, they found that the error was fixed from the complementary region of the corresponding healthy chromosome, rather than the supplied patch. Might this happening in your plants?
https://www.thenakedscientists.com/articles/science-news/gene-editing-corrects-embryonic-heart-defect
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