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Cells, Microbes & Viruses / Re: Viability of respiration-deficient yeast cells

« on: 01/04/2021 20:30:54 »

@Zer0

Hi, thank you for your reply! Yes, yeast can do both aerobic and anaerobic. But yeast prefers anaerobic even with the presence of oxygen. When all the sugar source is consumed, yeast cells can do aerobic (with the presence of oxygen) and convert alcohol into carbon dioxide and water.

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Cells, Microbes & Viruses / Viability of respiration-deficient yeast cells

« on: 22/03/2021 17:22:54 »

I use yeast cells and measured their chronological lifespan with SytoxGreen staining. If cells are dead, their DNA can be stained by SytoxGreen because the membranes are permeable. The stain was analyzed by flow cytometry.
In my experiments, I used respiration-deficient yeast cells (cyc3∆) and tested the percentage of the cells that were stained by SytoxGreen. The number dropped very quickly and reached 20% on day 25 of the culture. However, the number kept the same afterwards. When I ended my experiment on day 50 of the culture, 20% of the respiration-deficient cells were still 'alive', while they couldn't form any colonies on a plate.
I'm wondering why these cells could live such long. Mutation could be one of the reasons, and are there any other possibilities?

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Physiology & Medicine / Re: Is it possible that one’s gender is also biologically linked?

« on: 05/03/2021 17:21:26 »

I'm also curious about this question and will follow this page to see the answer from the others.

For me, I'm a bisexual girl and I think my experience built me. I had a very good girl friend in my childhood and we played a "hug and kiss game". Children usually don't judge and they accept anything they think is fun. I thought hugging and kissing a girl who I liked is fun. Now I still accept doing this.

However, it may also because my genes let me think "hugging and kissing a girl" is fun. In my opinion, there can be genes related to our emotion because emotion is based on neuron as well. If there are genes related to "acceptance" "human-loving" and so on, the combination could provide the possibility for people to be LGBT, given the environment to let them get in touch with the existence.