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Cells, Microbes & Viruses / Re: How do I calculate the cell density in a culture?

« on: 30/04/2022 03:19:03 »

Thank you so much!!! I totally get it!!!!!

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Cells, Microbes & Viruses / Re: How do I calculate the cell density in a culture?

« on: 29/04/2022 19:36:12 »

Thank you so much! I really appreciate it!
So one thing that's challenging to me is that these are suspension lines. I just need to plate them at an initial density that is not too low so that they won't proliferate. I'll be submitting them for proteomics and was told to make sure that the concentration for treated and untreated cells are fairly close when I harvest all the cells on Wed.

So I'll use the formula you suggest: Total # cells= Starting cell # * 2^ hrs. passed/doubling time
Lets say I want 4 million per ml for all the samples. How would I begin!
My attempt: 4,000,000= 2 to the power of 120/70. This would be for the 5 day? Can you check my calculation on this.
and then I'll subsequently do 4,000,000=2 to the power of 72/70 for the 3 day?

Thanks so much!!!

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Cells, Microbes & Viruses / Re: How do I calculate the cell density in a culture?

« on: 29/04/2022 02:56:11 »

Thanks a lot! OK so I get the fact that Cell X will have 2.4 doublings and Cell Y will have 3.2 doublings

From here I'm lost! What's the equation that I would need to use to figure out the plating cell # that takes into account the # of doublings, time in culture etc! That's what I'm confused about! This is what happens when you graduate from a community college in the US!

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Cells, Microbes & Viruses / How do I calculate the cell density in a culture?

« on: 28/04/2022 21:46:21 »

Hi Guys-

I'm new to Tissue culture and have a basic question.

I have 2 cell lines: Line X doubling time=50 hr and Line Y doubling time=37 hrs.



My question is that I want to do a 5 day tmnt with a drug and harvest all the samples at the same time. So for example, I'll start my 5 day tmnt. on a Fri etc--see below. And harvest all the samples on the following Wed. My issue is that I need to maintain similar density for ALL samples--how do I calculate what density I should plate all the tmnt samples so that when I harvest cells on Wed--the data won't be confounded by cell density? Thanks so much!!!!






Day   Tmnt.Time   Start tmnt
Fri   d0   5d   12pm
Sun   d2   3d   12pm
Tues   d4   1d   12pm
Wed   d5   3hr   9am
Wed   d5   Ohr   12pm