Hi Guys-

I'm new to Tissue culture and have a basic question.

I have 2 cell lines: Line X doubling time=50 hr and Line Y doubling time=37 hrs.



My question is that I want to do a 5 day tmnt with a drug and harvest all the samples at the same time. So for example, I'll start my 5 day tmnt. on a Fri etc--see below. And harvest all the samples on the following Wed. My issue is that I need to maintain similar density for ALL samples--how do I calculate what density I should plate all the tmnt samples so that when I harvest cells on Wed--the data won't be confounded by cell density? Thanks so much!!!!






Day   Tmnt.Time   Start tmnt
Fri   d0   5d   12pm
Sun   d2   3d   12pm
Tues   d4   1d   12pm
Wed   d5   3hr   9am
Wed   d5   Ohr   12pm