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Life Sciences => Physiology & Medicine => Topic started by: suvaleen on 03/10/2005 10:33:21

Title: What is Turk's fluid and how it is used to calculate total leucocyte count?
Post by: suvaleen on 03/10/2005 10:33:21

Hi friends,can you pls explain me what is Turk's fluid which is used in Total Leucocyte count.[?]And what is the function?

suva

Title: Re: What is Turk's fluid and how it is used to calculate total leucocyte count?
Post by: iko on 21/06/2008 16:22:59

Hi Suvaleen,

I know it's too late, and you probably found the answer somewhere else.
Turk's solution has a stain (gentian violet) plus acetic acid 6%:
diluting blood 1:20 with this solution, white cells' nuclei are more visible, stained in blue, red cells are destroyed by hypotonic solution plus acetic acid.
A drop can be placed in a fixed-volume glass "chamber" and observed under the microscope for counting and calculating white blood cell concentration.

http://iai.asm.org/cgi/reprint/36/1/325.pdf

...boring and time consuming method of the past: today we fortunately use automatic analyzers!  [8D]

(https://www.thenakedscientists.com/forum/proxy.php?request=http%3A%2F%2Fwww.fao.org%2FDOCREP%2F003%2FW3732E%2Fw3732e0k.gif&hash=ad7f0209d94e55a5ed4bd432613115a3)
http://www.fao.org/DOCREP/003/W3732E/w3732e0k.gif

http://www.fao.org/DOCREP/003/W3732E/w3732e0b.htm