Naked Science Forum

Non Life Sciences => Chemistry => Topic started by: MarianaM on 11/09/2019 13:27:57

Title: Will saline lower the absorbance values if used to dilute BSA?
Post by: MarianaM on 11/09/2019 13:27:57
Lucida asks...

If saline is used to dilute BSA instead of water for a standard, will saline lower the absorbance values?

Can you help?
Title: Re: Will saline lower the absorbance values if used to dilute BSA?
Post by: Bored chemist on 11/09/2019 18:52:24
It's impossible to say without testing under exactly the circumstances you are making the measurements. At the very least, there's not enough information to answer the question.

If you want to be sure you have the right answers, use the right diluent.