Naked Science Forum

Life Sciences => Physiology & Medicine => Topic started by: hamidicup on 06/11/2012 19:43:57

Title: Histology/Immunology
Post by: hamidicup on 06/11/2012 19:43:57

Hello Naked Scientists!

I was wondering, how to induce inflammation in rabbits? As i am going to study the anti-inflammatory activity of certain plants.

Title: Re: Histology/Immunology
Post by: CliffordK on 06/11/2012 23:40:32

I would assume you could acquire various antigens from common pathogens. 

For example various mold antigens such as candida and aspergillus antigens could cause inflammation without infection.

In humans, why do slivers cause such inflammation?  Perhaps you could even try a sterile foreign object such as a wood sliver.

And, of course, actual pathogens will cause inflammation

The last time I pounded my thumb with a hammer, I noticed moderate inflammation, including the thumb being about 10 °F warmer than the other thumb.

You might wish to look at the TB skin test in humans.  Obviously it is unlikely that the rabbits have been exposed to human TB, but the idea might be the same, using something that the rabbits have been exposed to.

Title: Re: Histology/Immunology
Post by: RD on 07/11/2012 02:48:51

http://en.wikipedia.org/wiki/Animal_testing_regulations

Title: Re: Histology/Immunology
Post by: hamidicup on 07/11/2012 06:15:30

ThankS Clifford such a nice idea..

Title: Re: Histology/Immunology
Post by: CliffordK on 07/11/2012 08:42:29

Quote from: RD on 07/11/2012 02:48:51

http://en.wikipedia.org/wiki/Animal_testing_regulations

Ok, so depending on the goals, perhaps mice might also be a good choice for test animals.

While there are many types of injuries that might cause inflammation, one may choose the least painful type of insult that will lead to the desired results.  And, perhaps use anaesthesia during any potentially painful procedures.

And, the test procedures still need to be planned, documented, and approved.