Naked Science Forum

Life Sciences => Cells, Microbes & Viruses => Topic started by: Gwhite1 on 24/04/2017 01:40:44

Title: Is there a way to lyse both red cells and white cells but not lyse bacteria?
Post by: Gwhite1 on 24/04/2017 01:40:44

How can one lyse RBC And WBC but not lyse bacteria, particularly gram negative.  ACK, dilute acid, and hypotonic solutions rapidly lyse RBC.  I see videos on YouTube of water lysing both RBC and WBC, though it seems to take longer for WBC.  Is there a good way to lyse both RBC and WBC but not lyse gram negative bacteria?

Title: Re: Is there a way to lyse both red cells and white cells but not lyse bacteria?
Post by: chris on 24/04/2017 18:12:05

Bacteria, being equipped with a cell wall, will be a lot more resistant to hypotonic solutions that blood cells.

Title: Re: Is there a way to lyse both red cells and white cells but not lyse bacteria?
Post by: Gwhite1 on 25/04/2017 01:08:09

Does anyone have a recommended procedure?  What Diluant, what ratio, how much time to luse the WBC but not harm gram negative bacteria.

Title: Re: Is there a way to lyse both red cells and white cells but not lyse bacteria?
Post by: MayoFlyFarmer on 12/05/2017 15:44:19

I've never tried, so I don't have an exact protocol, but I think you're spot on with water.  Most bacteria hold up quite well in water, while WBCs should lyse quite rapidly.