Naked Science Forum
Life Sciences => Cells, Microbes & Viruses => Topic started by: Gwhite1 on 24/04/2017 01:40:44
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How can one lyse RBC And WBC but not lyse bacteria, particularly gram negative. ACK, dilute acid, and hypotonic solutions rapidly lyse RBC. I see videos on YouTube of water lysing both RBC and WBC, though it seems to take longer for WBC. Is there a good way to lyse both RBC and WBC but not lyse gram negative bacteria?
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Bacteria, being equipped with a cell wall, will be a lot more resistant to hypotonic solutions that blood cells.
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Does anyone have a recommended procedure? What Diluant, what ratio, how much time to luse the WBC but not harm gram negative bacteria.
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I've never tried, so I don't have an exact protocol, but I think you're spot on with water. Most bacteria hold up quite well in water, while WBCs should lyse quite rapidly.