Naked Science Forum
On the Lighter Side => Science Experiments => Topic started by: Mrak.B on 02/05/2010 18:20:16
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I need someone to help me how to do backcrossing in fission yeast S.pombe ( the steps) to confirm that the phenotype is linked to the mutation has been done by knocking out a gene and replace it by Kanmx gene. I 'll be grateful if someone refer to me an article or books to learn from as well.
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I found this
http://www.cell.com/retrieve/pii/009286749390164L
and this
http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe
and this
http://jvi.asm.org/cgi/content/full/74/6/2636