Naked Science Forum

Non Life Sciences => Physics, Astronomy & Cosmology => Topic started by: scientizscht on 05/11/2018 20:37:04

Title: What affects rates of electrophoresis?
Post by: scientizscht on 05/11/2018 20:37:04

hello!

What affects the rate of movement in electrophoresis?

I suppose larger charges move faster, but what happens with weight/volume?

Thanks!

Title: Re: What affects rates of electrophoresis?
Post by: chris on 06/11/2018 07:44:18

Electrophoretic mobility is proportional to the density of the gel being used, the applied potential difference, the size(s) of the fragments being separated and the charge on those fragments.

Title: Re: What affects rates of electrophoresis?
Post by: scientizscht on 06/11/2018 08:36:51

Quote from: chris on 06/11/2018 07:44:18

Electrophoretic mobility is proportional to the density of the gel being used, the applied potential difference, the size(s) of the fragments being separated and the charge on those fragments.

So larger go slower?

Title: Re: What affects rates of electrophoresis?
Post by: chris on 06/11/2018 14:13:58

In general, yes; the larger molecule is harder for the field to drag through the gel; but this may be balanced by the charge on the fragment. So a large but highly charged protein species could still move more than a smaller species with fewer charges, for instance.