Naked Science Forum

Non Life Sciences => Geek Speak => Topic started by: Lewis Thomson on 12/01/2022 10:19:30

Title: Why do SPAM probes boost the signal of a qPCR?
Post by: Lewis Thomson on 12/01/2022 10:19:30

Montse has sent us an email with this question attached.

"Does anybody have an idea of what's the reason why SPAM probes (and also other double processed meats) increase the endpoint signal of the qPCR? Because there's an explanation of why it decreases background hunger, but not of why increases the waistline."

Can you help? Leave you points in the comments below...

Mod edit: Specific brand names removed

Title: Re: Why do SPAM probes boost the signal of a qPCR?
Post by: evan_au on 12/01/2022 20:34:36

A high-level description of quenching in PCR testing is available here:
https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction#Chemical_classification