help me with extractions please..

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Offline mordoh

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help me with extractions please..
« on: 14/06/2004 12:20:36 »
Hello..

please help me, i need to extract flavonoids from plants
do you know of a simple protocol that i can seperate them from the plant tincture,

i also need to know how (if i cant seperate the flavonoids) i can get rid of un needed substance in the plant - like wax, oils etc .. - i heard about hexan, chloroform (are they toxic?) and Naphtha

also if you can help me by giving me a protocol of isolating catechins from green tea

one more question, what is the diffrence between extraction of herb with acetone and with ethanol ?

thanks alot , i really need answers.
 

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Offline Titanscape

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Re: help me with extractions please..
« Reply #1 on: 14/06/2004 17:42:23 »
Perhaps grinding and centrfuge work or freezing or boiling. Or even cold vaporization in a large horse syringe.

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Offline Ylide

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Re: help me with extractions please..
« Reply #2 on: 16/06/2004 05:55:12 »
Ethanol extraction would be a good place to start.  It depends on what you're trying to extract, every plant has flavonoids of differing chemical composition.  Most of those types of compounds are soluble in alcohols, and ethanol is the least toxic to your body.  (if you're going to be consuming them)  It also has the benefit of not being a good solvent for oils and waxes, so you can leave behind most of the crud.  

Acetone, if you substance is soluble in it, is good because it's really easy to evaporate out of the mix entirely, leaving you with dry product.  



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