0 Members and 1 Guest are viewing this topic.
TESTING TECHNIQUES. Urine samples are tested with a variety of different instruments and techniques.Some tests use dipsticks, which are thin strips of plastic that change color in the presence of specific substances.Dipsticks can be used to measure the acidity of the urine (its pH) or the presence of blood, protein, sugar
I suspect that those strips only respond to glucose and are "blind" to sucrose.Having said that, boiling the drink with dilute sulphuric acid for a while, neutralising it and then measuring the glucose formed by the hydrolysis of the sucrose might work.You would need to do a test with known solutions of sugar in water.I'd be looking really hard at a density measurement- perhaps with a titration to measure the acidity and then correcting for that.
Sucrose itself is a non-reducing sugar, having the reducing aldehyde group involved in the glycosidic bond, and therefore will not test positive with Benedict's solution. In order to test for sucrose, the enzyme sucrase is mixed with the sample. The sucrose is hydrolysed into glucose and fructose, with glucose being a reducing sugar, which will test positive with Benedict's solution. Fructose can be isomerized to glucose by the addition of NaOH or other strong base.