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  4. What is Turk's fluid and how it is used to calculate total leucocyte count?
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What is Turk's fluid and how it is used to calculate total leucocyte count?

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Offline suvaleen (OP)

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What is Turk's fluid and how it is used to calculate total leucocyte count?
« on: 03/10/2005 10:33:21 »
Hi friends,can you pls explain me what is Turk's fluid which is used in Total Leucocyte count.[?]And what is the function?

suva
« Last Edit: 21/06/2008 18:42:09 by chris »
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Offline iko

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Re: What is Turk's fluid and how it is used to calculate total leucocyte count?
« Reply #1 on: 21/06/2008 16:22:59 »
Hi Suvaleen,

I know it's too late, and you probably found the answer somewhere else.
Turk's solution has a stain (gentian violet) plus acetic acid 6%:
diluting blood 1:20 with this solution, white cells' nuclei are more visible, stained in blue, red cells are destroyed by hypotonic solution plus acetic acid.
A drop can be placed in a fixed-volume glass "chamber" and observed under the microscope for counting and calculating white blood cell concentration.

http://iai.asm.org/cgi/reprint/36/1/325.pdf

...boring and time consuming method of the past: today we fortunately use automatic analyzers!  [8D]



http://www.fao.org/DOCREP/003/W3732E/w3732e0k.gif

http://www.fao.org/DOCREP/003/W3732E/w3732e0b.htm
« Last Edit: 23/06/2008 21:36:24 by iko »
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