Spectrophotometry question

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Offline dinesh

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Spectrophotometry question
« on: 05/11/2005 21:37:06 »
I did a biochem lab on absorbance , using ninhydrin with Tyrosine and glycine and then measuring their absorbance  @ 570 nm
My absorb for Tyro was at around .557 , and Glycine at around .623 .

What accounted for this?
I know that tyro has the bezene ring, with its delocalized electrons ..but can anyone help me with a better explaination?

THanks.
 

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Offline Ylide

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Re: Spectrophotometry question
« Reply #1 on: 11/11/2005 11:46:41 »
Tyrosine absorbs more in the UV spectrum.  Measure absorbance around 280nm and you'll see a big difference.  



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