RNAase cut RNA to the same length fragments??

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Offline vj_tu

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RNAase cut RNA to the same length fragments??
« on: 15/01/2006 21:19:29 »
I carried out Agarose Gel electrophoresis of RNA from yeast cut with RNAse with varied concentrations in each well and it turned out that there is only one band in each well of the gel,in contrary,the electrophoresis of DNA from yeast cut with endonuclease produced several bands. Do you have explanation for the difference? How can RNAse which is endonuclease enzyme cut off RNA molecule to fragments at the same lenght to produce only one band?


Offline rosy

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Re: RNAase cut RNA to the same length fragments??
« Reply #1 on: 16/01/2006 00:04:27 »
It will depend on exactly what RNAse/DNAse you're using, won't it? Did you get a load of stuff left in the well too? If you're using an RNAse which cuts very rarely, for example (typically one which is highly specific for a fairly long stretch of code) then you could easily get only one band moving through the gel (the other sections being much longer and remaining unresolved in the well).
Or I guess you could be using a frequent cutter and there happens to be one stretch that isn't cut and so is left to make a band in the gel and the rest runs off the top?
I'm trying to remember what would be reasonable, I haven't studied any biochemistry for a couple of years now and I wasn't really paying attention when I did, so I could be way off... and I'm not very clear about what the experiment was in the first place.
Anyone who knows anything care to have a go??