RFLP alleles

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Offline vj_tu

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RFLP alleles
« on: 20/01/2006 19:03:04 »
Can we use Northern Blotting,Acrylamide gel electrophoresis and PCR to detect RFLP alleles of 2.5 kbp and 5kbp or 7.5 kbp of a human chromosome 4 locus??
 

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Offline chris

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Re: RFLP alleles
« Reply #1 on: 21/01/2006 11:27:55 »
I'm not sure why you'd want to use Northern blotting - that's for RNA. It would be more sensible to amplify the region by PCR and look at the band sizes.

Alternatively you'd perform a restriction digest, electrophorese the products on agarose and then perform a southern blot probing the gel with the region common to all alleles.

Chris

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I never forget a face, but in your case I'll make an exception - Groucho Marx