Suggestion for use of internal control in gene experssion study in e.coli

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Offline sambiotech

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hello all.

can any one provide me details about what house keeping gene should i use as internal control for studying TEM and HSV genes experssion study in e.coli. These genes are Beta Lactamase producing genes responsible in Efflux pump action of Beta lactum antibiotics.

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Offline Devilmunkey

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I don't really know enough about the genetic structure of E. coli to make a knowledgable contribution. But perhaps a mitrochondrialy expressive gene may prove suitable as ATPase genes are relatively ubiqitious, I beleive.

I apologise if this is erroneous, it was just a thought...
« Last Edit: 11/01/2012 23:18:16 by Devilmunkey »

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Offline virologist85

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Hey Sam,

Hopefully you've figured out a control by now... but just in case, you could try a ribosomal gene. I use those for controls in eukaryotic cell experiments and they work pretty well.

Best of luck.

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Offline Koblenz

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Hello,

I hope you have figured this out now :)

Common housekeeping genes used as controls are recA, rpoB, tuf... Any paper on gene expression regulation would give you the details of the genes and the related primers.