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quote:Dopamine (DA) is regarded as an important modulator of enteric function. Recent experiments have suggested that newly cloned DA receptor subtypes are widely expressed in peripheral organs, including the gastrointestinal tract. In the present studies, the D1A receptor subtype was identified in rat gut regions through localization of receptor protein by means of light microscopic immunohistochemistry and Western blot analysis and receptor mRNA by RT-PCR and in situ amplification and hybridization (3SR in situ). D1A receptor immunoreactivity was shown to have a diverse distribution in the gastrointestinal tract, being present in the gastroesophageal junction, stomach, pylorus, small intestine, and colon. The receptor has a transmural distribution present in both epithelial and muscle layers as well as in blood vessels and lamina propria cells of different gastrointestinal regions. Western blot analysis demonstrated a single 50-kDa band for esophagus, stomach, duodenum, jejunum, and colon. The in situ hybridization signal was localized to the same sites revealed by D1A receptor immunoreactivity. RT-PCR revealed an appropriate sized signal in similar regions. This study is the first to identify expression of the central D1A receptor throughout the normal mammalian gastrointestinal tract. immunohistochemistry; messenger ribonucleic acid
quote:(Serotonin is made in the intestines, which is also where 95% of the body's supply is located -- most of the rest is in the brain, with trace amounts in the blood and other organs.)
quote:Originally posted by geckoi think the spiritual feelings you may get from fasting are a result of your relating fasting to spirtuality. if you expect to feel that way, you will.