Effect of pH on DNA backbone? (biochemistry)

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Offline Patty

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Effect of pH on DNA backbone? (biochemistry)
« on: 19/09/2006 02:36:49 »
Can hydrogen ion concentration (low pH) break the DNA backbone? Can it do it in a sequence-specific manner under any conditions?
 

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Offline Patty

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Re: Effect of pH on DNA backbone? (biochemistry)
« Reply #1 on: 21/09/2006 23:18:13 »
I've found a few articles that indicate this might happen, but nothing conclusive. So I'm still wondering if anyone has any knowledge about this.

I believe that the seqience specificty we see may be the resul;t of inverted repeats in the DNA< causing the DNA to assume a cruciform conformation with unpaired bases in the loops. Still, we're at a bit of a loss to explain why the DNA is cleaving in our samples.
 

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Offline Nanac

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Re: Effect of pH on DNA backbone? (biochemistry)
« Reply #2 on: 22/09/2006 07:23:53 »
Extracting the patented technique is not so easy .