Determination Of Azo Dye ?

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Offline Chem4u

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Determination Of Azo Dye ?
« on: 16/03/2015 19:38:42 »
Hello Every One
I want To Know how The Determination Of Azo dye By Spectrophotometer>>
I want
1- Theory
2- Equation
Thx  In Advanced

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Offline chiralSPO

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Re: Determination Of Azo Dye ?
« Reply #1 on: 16/03/2015 19:47:04 »
I can only guess you are probably looking for Beer's law...

http://www.chm.davidson.edu/vce/spectrophotometry/beerslaw.html
http://en.wikipedia.org/wiki/Beer%E2%80%93Lambert_law (not easy to understand)

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Offline evan_au

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Re: Determination Of Azo Dye ?
« Reply #2 on: 16/03/2015 20:12:23 »
I heard a very amusing description of the accidental discovery of azo dyes, on the BBC's "The Infinite Monkey Cage", with Brian Cox.
 
Listen to: http://downloads.bbc.co.uk/podcasts/radio4/timc/timc_20150223-1700a.mp3
The story starts at 5:45, and runs to 11:45 (but the whole episode on "Serendipity" is quite good).

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Offline Chem4u

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Re: Determination Of Azo Dye ?
« Reply #3 on: 16/03/2015 20:27:47 »
Thanks All ^_^