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  4. Is there a way to lyse both red cells and white cells but not lyse bacteria?
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Is there a way to lyse both red cells and white cells but not lyse bacteria?

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Offline Gwhite1 (OP)

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Is there a way to lyse both red cells and white cells but not lyse bacteria?
« on: 24/04/2017 01:40:44 »
How can one lyse RBC And WBC but not lyse bacteria, particularly gram negative.  ACK, dilute acid, and hypotonic solutions rapidly lyse RBC.  I see videos on YouTube of water lysing both RBC and WBC, though it seems to take longer for WBC.  Is there a good way to lyse both RBC and WBC but not lyse gram negative bacteria?
« Last Edit: 24/04/2017 18:11:04 by chris »
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Offline chris

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Re: Is there a way to lyse both red cells and white cells but not lyse bacteria?
« Reply #1 on: 24/04/2017 18:12:05 »
Bacteria, being equipped with a cell wall, will be a lot more resistant to hypotonic solutions that blood cells.
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Re: Is there a way to lyse both red cells and white cells but not lyse bacteria?
« Reply #2 on: 25/04/2017 01:08:09 »
Does anyone have a recommended procedure?  What Diluant, what ratio, how much time to luse the WBC but not harm gram negative bacteria.
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Re: Is there a way to lyse both red cells and white cells but not lyse bacteria?
« Reply #3 on: 12/05/2017 15:44:19 »
I've never tried, so I don't have an exact protocol, but I think you're spot on with water.  Most bacteria hold up quite well in water, while WBCs should lyse quite rapidly.
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Tags: bacteria  / red cells  / white cells  / blood cells  / cell lysis 
 

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