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Author Topic: NATIVE PAGE  (Read 2403 times)

Offline vamseedhar_r

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NATIVE PAGE
« on: 22/01/2006 08:44:08 »
Can we determine the molecular weight of a protein using NATIVE-PAGE? If no, then what are its other uses?
Also, does the usage of NATIVE PAGE depend on the isoelectric point of the protein being used? If a protein's overall charge is +ve, then where will it move during a NATIVE gel run?


 

Offline nilmot

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Re: NATIVE PAGE
« Reply #1 on: 22/01/2006 16:37:46 »
NATIVE PAGE is a protein separation method that depends on the mass to charge ratio of the molecule. As long as the molecule is charged it will move, the direction of movement of the molecule depends on which way you run the electrophoresis i.e. +ve to -ve or vise versa.

Isoelectric focusing is another method of protein separation that has a pH gradient across the gel and separation depends on the isoionic point (pI) of molecule, i.e. it will move to the point where the pH is equal to its pI.

As to determine the Mr err I'm not sure.

Tom
 

Offline Ray hinton

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Re: NATIVE PAGE
« Reply #2 on: 02/02/2006 23:12:55 »
oh dear
i came here cos i thought this page was about indiginous peoples.

every village has one !
 

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Re: NATIVE PAGE
« Reply #2 on: 02/02/2006 23:12:55 »

 

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