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Technology / Re: What are the methods to reverse a rotation?
« on: 17/01/2021 17:28:19 »
I can't get my head around.
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Thanks but if you have a rotating shaft, can you put an addon on the shaft so that you get a reverse rotation?Did you not like the answer?gears, wheels, other simple machines for direct mechanical transfer.
Is it better to use Mass spec?Probably not in this case.
Do you have or can you get gluconic acid?
Are you using a mass spec detector?
Well... how do you measure ("see") your glucose on the hplc? If you're using a mass spec detector, please stop injecting phosphate buffer into it.
The first thing I would do would be google it and see if someone has done it before.
Something like this
https://www.shodexhplc.com/applications/lc-ms-analysis-of-glucose-and-gluconic-acid-vg-50-2d/
The other thing I would do would get a sample of the gluconic acid.
That way I could measure it as well as measuring glucose.
If, for example, 1% of your glucose is oxidised, it will be difficult to measure the change in concentration- especially as there will be some evaporation when the material is exposed to the air.
But if 1% of the glucose if converted to the acid, you will see the acid easily.
I have not been able to find online whether HPLC can distinguish between glucose and gluconate.Well, it would if I was doing it but it seems that you don't know how, because, if you did, you would have checked that first.
no glucose reactedHow do you know?
But, the most likely answer is that diffusion from the air into your reaction vessel was too slow.
Did you put the solution in a shallow flat tray, a beaker or a conical flask?
Obviously, with no stirring, the reaction is going to be much slower further from the surface.
So the "rate of reaction" isn't going to be well defined, is it?
What are you actually trying to find out?
The question is, will oxygen diffuse from air into the waterObviously, yesLife is one of those "reactions" that occurs in water.A lot of "life" involves the enzymatic oxidation of glucose.
But you didn't want that answer either.
The question is simple: will oxygen impede the glucose oxidation as it is three magnitudes smaller?And the answer is also simple.It depends.
Any indications?No, you have not provided any indications.
Would you like to, or is this another of your posts where you ask a stupidly open question then get upset when it's impossible for anyone to answer.
I need to know whether oxygen is the limiting factor in oxygen reduction for oxygen-using oxidising enzymes in water solutions.It depends.