1
Chemistry / calculation problem in the preparing of phosphate buffer
« on: 31/07/2007 14:38:10 »
i have NaH2PO4,molecular weight is 156.01 and the NaOH with 1.0M...
if i want to prepare 25mM phosphate buffer, pH7.4
how much of the solutions i have to use to add up the total volume is 100mL?
after prepare the phosphate buffer,i need to add 1.0mM EDTA (MW = 292.25 ), 0.1mM PMSF (MW = 174.19)and 0.1mM DTT (MW = 154.25) into the phosphate buffer to add up until 50mL...
how much of EDTA, PMSF and DTT i need to add in to the phosphate buffer?
if i want to prepare 25mM phosphate buffer, pH7.4
how much of the solutions i have to use to add up the total volume is 100mL?
after prepare the phosphate buffer,i need to add 1.0mM EDTA (MW = 292.25 ), 0.1mM PMSF (MW = 174.19)and 0.1mM DTT (MW = 154.25) into the phosphate buffer to add up until 50mL...
how much of EDTA, PMSF and DTT i need to add in to the phosphate buffer?