[Mrak.B (OP)]

I need someone to help me how to do backcrossing in fission yeast S.pombe ( the steps) to confirm that the phenotype is linked to the mutation has been done by knocking out a gene and replace it by Kanmx gene. I 'll be grateful if someone refer to me an article or books to learn from as well.

[Jolly- Joliver]

I found this
http://www.cell.com/retrieve/pii/009286749390164L

and this

http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe

and this

http://jvi.asm.org/cgi/content/full/74/6/2636