Actual Kitchen Science - Plant Micropropagation

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Offline MarkPawelek

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Actual Kitchen Science - Plant Micropropagation
« on: 31/10/2013 22:49:20 »
Plant Tissue Culture: Micropropagation [nofollow] (youtube)

Plants from Test Tubes, 4e [nofollow] (3rd ed is good too)

You don't need a laminar flow hood. You can use a DIY glove compartment :- a big box (ply-wood or even cardboard, with a perspex front).

The most important thing you need is sterility, steady hands and patience. Clean your kitchen spotless, with a bleach spray. A UV-light is good too.

It should be easy to order the plant nutrients from a chemical supplier (even in this age of home drug and terrorist lab paranoia!).

Essentials: Pressure cooker (home 5L size is OK), DIY glove compartment, alcohol or gas lamp, jars. Scalpel with replaceable blades and forceps (use proper blades and forceps from your supplier as these are very cheap).


Offline CliffordK

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Re: Actual Kitchen Science - Plant Micropropagation
« Reply #1 on: 10/11/2013 08:49:41 »
Your video has a wicked echo.

Is this a "tissue culture" or a "seed culture".  At 2:30 in the film, it talks about treating seeds, and later it talks about recording the growth of seedlings.  Perhaps the lab setting has a bit higher germination rate that I get outside, but it does seem very much seed based.  Does the process depend on the type of seed?

It might be interesting adapting the process to a home environment.  Perhaps sterilizing equipment in an oven.  Perhaps you could use simple glass jars (near the end, they show removing a plant from a jar rather than a flask). 

Does everything really need to be so sterile?  Perhaps a fungicide would be helpful, although many seeds naturally have yeasts.

As far as propagation, I've been experimenting with Air layering, and am hoping to try some grafting next spring.