When transcribing DNA how do we know which way round it is?

  • 1 Replies

0 Members and 1 Guest are viewing this topic.


Offline thedoc

  • Forum Admin
  • Administrator
  • Sr. Member
  • *****
  • 513
    • View Profile
Peter Palmer asked the Naked Scientists:
Since both DNA strands have a 3' end, and I know of no means a cell can differentiate sense from anti-sense, (other than potentially distance to the centromere), is there a reason a reverse complement incorporating the combined promoter and starter sequences is unlikely, in order to avoid spurious primary transciptions?

What do you think?
« Last Edit: 29/09/2015 08:50:01 by _system »


Offline evan_au

  • Neilep Level Member
  • ******
  • 4309
    • View Profile
Reading complementary DNA is likely to end up hitting a "STOP" codon fairly quickly?
...But this would still leave a lot of short junk RNA sequences floating around...